| Summary: In this thesis, the in vitro production of Orchis sancta L. and Serapias vomeracea (Burm. F.) Briq., belonging to the Orchidaceae family, acclimatization of in vitro produced plantlets as well as production and germination of their synthetic seeds have been studied. Orchimax including activated charcoal was the best germination medium for the mature seeds of O. sancta and S. vomeracea. The highest germination rates were 81.18% and 84.03% for O. sancta and S. vomeracea, respectively when they were individually cultured on Orchimax medium supplemented with 1.0 mg/L ZEA and 2.0 mg/L 6-BA. The medium containing 0.25 mg/L TDZ gave the superior shoot elongation in O. sancta and S. vomeracea with the values of 53.24 mm and 42.88 mm, respectively. The most effective leaf formation was obtained from O. sancta cultures maintained in the medium supplemented wih 1.0-2.0 mg/L KIN whilst that of S. vomeracea was 2.0 mg/L IBA. Amongst all plant growth regulator concentrations tested, high concentrations of IBA enhanced the root formation and elongation for both species. The highest corm formation as well as their glucomannan contents extracted from in vitro growing O. sancta and S. vomeracea plantlets were detected in the medium supplemented with 2.0 mg/L ZEA. Plantlets of O. sancta and S. vomeracea were carefully taken from their growth media and successfully acclimatized in the soil. Synthetics seeds of O. sancta and S. vomeracea were prepared and succefully germinated in growth medium and also in commercially available torf medium. |
