Ph.D. Tezi Görüntüleme

      Iridoviruses have a wide host range including fish, amphibians, reptiles and invertebrates (especially insects), which have ecological and economic significance. Determining the interactions between structural viral proteins is important for the understanding of virus maturation, infection and release mechanisms. In this study, 2209 possible interactions between 47 structural proteins of Chilo iridescent virus have been investigated by yeast-two-hybrid system. The structural genes have cloned in both bait and prey vectors. Bait vector was introduced into Saccharomyces cerevisiae AH109. Expression of the bait genes was confirmed by western blot hybridisation analysis. After confirmation, prey vectors were transformed into yeast cells, which carry the bait vectors. Yeast cells contain bait and prey vectors were grown on a selective medium (minimal synthetic defined) to determine the protein-protein interactions between BAIT and PREY proteins. The colonies grown on this medium indicate positive interactions between respective proteins. 163 interactions were determined among 47 structural proteins. The interactom scheme has drawn by using cytoscape program. The results of this study will provide useful information for the understanding of infection, assembly and egress mechanisms of the virus.