Ph.D. Tezi Görüntüleme

Student: Handan KARAOĞLU
Supervisor: Assoc. Prof. Bilal KUTRUP
Department: Biyoloji
Institution: Graduate School of Natural and Applied Sciences
University: Karadeniz Technical University, Turkey
Title of the Thesis: Acute and Chronic Effects of Ammonium nitrate Fertilizer on Caucasian Parsley Frog (Pelodytes caucasicus) Tadpoles
Level: Ph.D.
Acceptance Date: 21/1/2011
Number of Pages: 133
Registration Number: Di820
Summary:

      The Pelodytes caucasicus, endemic Caucasian Isthmus and inhabit only in the Eastern Black Sea Region of our country, has been included in the Red List of Threatened Species as near threatened by the IUCN (The International Union for Conservation of Nature) and ammonium nitrate is the most widely used fertilizer that the main reason leading to recent significant decrease in the amphibian population throughout the world. There are no studies conducted on the effects of ammonium nitrate on the Pelodytes caucasicus species in Turkey or in the world.

Within the scope of this study, concentrations of ammonium nitrate (0, 5, 10, 15, 20, 25 mg/L) that can be found in nature have been applied chronically for 70 days and very high concentrations (0, 50, 75, 100, 150, 250, 500 mg/L) have been acutely applied for 15 days to 10 days old Pelodytes caucasicus larvae hatched in the laboratory from eggs collected from nature. Furthermore, the test was conducted on two different populations, of which one was obtained from an unpolluted area and the other from a polluted area, in order to determine whether or not there was difference in their level of resistance to ammonium nitrate. Accordingly, parameters such as the growth rates, percentages and times of anomaly and mortality of these populations and LC50 values that lead to the mortality of half of the populations were studied.

      In the light of the obtained results, it has been determined that ammonium nitrate fertilizer decreased growth and caused various behavioral and physical anomalies and also mortality in both populations at both chronic and acute levels. It has been determined that, cases of mortality and anomaly increased in parallel with increases in concentration and time and however, both cases occurred far higher (p≤ 0,05) in the population obtained from unpolluted areas in comparison to those obtained from polluted areas and these cases commenced significantly earlier in the population from unpolluted areas. Furthermore, the LC50 value has been determined to be 170 mg/L for the unpolluted area population and 204 mg/L for the polluted area population. As a result of these data, it is thought that an adaptation that increases resistance against ammonium nitrate has developed in the polluted area population.

      Key words: Ammonium nitrate, Pelodytes caucasicus tadpoles, acute and chronic effects