M.Sc. Tezi Görüntüleme

      In the Turkish population with inflammatory bowel diseases, the mutation rate of 3020 insC of CARD15 /NOD2 gene was IBD 28.9 %, CD 38 % , and UC 25% and the related rate was 4 % concerning the healthy individuals.When compared with the cytokine genes of healthy individuals, the frequency of cytokine gene polymorphism in inflammatory bowel diseases increased in genes IL-1 RA, IL- 4RA, TGF&#946; and TNF&#945; (p < 0.05) but decreased in genes IL -1&#945; , IL-1&#946; , IL- 12 , IFN &#947; , IL -2, IL- 4, IL- 6 and IL -10 (p< 0.05); in Crohn diseases it increased in genes IL-1, IL-4RA, and TNF&#945; of (p < 0.05) but decreased in genes IL-1, IL-4RA, IFN-&#947;, TGF&#946;, TNF, IL-2, IL-4, IL-6 and IL-10; in Ulcerative colitis it increased in genes IL &#946;, IL- 1 RA, IL- 4RA, IL-12, TGF&#946; and TNF&#945; (p< 0.05) but decreased in genes IL-1&#945; , IFN &#947;, IL-2 , IL-4, IL-6 and IL-10 (p< 0.05); there was no difference in other genes.In sum, in the Turkish population the mutation of 3020 insC of CARD15/NOD2 gene is not unique to Crohn disease, but this mutation is present in Ulcerative colitis as well.We are of the opinion that this mutation is not itself the only determining factor for clinical diagnoses; however, it can be used in the clinical diagnosis of IBD in order to determine the low level or high level variations in cytokine gene polymorphism.

      Keywords: Inflammatory Bowel Disease, Crohn Disease, Ulcerative Colitis, CARD15/NOD2 Gene, Cytokine Gene Polymorphism, PCR-SSP, Specific Multiplex PCR