M.Sc. Tezi Görüntüleme

Student:	Özlem BÜLBÜLOĞLU
Supervisor:	Assoc. Prof. Dr. Zihni DEMİRBAĞ
Department:	Biology
Institution:	Graduate School of Natural and Applied Sciences
University:	Karadeniz Technical University, Turkey

Title of the Thesis:	ISOLATION AND CHARACTERIZATION OF BACILLUS THURINGIENSISES FROM VARIOUS SOIL SAMPLES AND DETERMINING INSECTICIDAL EFFECTS OF THEM
Level:	M.Sc.
Acceptance Date:	28/8/2000
Number of Pages:	40
Registration Number:	i1154

Summary:

In this study, isolation of B. thuringiensis from various soil samples and identification of these bacteria have been carried on. Also, insecticidal efects of these isolates were identified on insects belong to Lepidoptera, Hymenoptera and Coleoptera.

Eighty five isolates were obtained from soil samples taken from three different places. Among these bacteria, 40 isolates were detected as B. thuringiensis according to morphological and biochemical test results. At the end of molecular tests that were done with ISR (Intergenic 16S-23S Spacer Region) assay, it was determined that 28 isolates hassame properties as the control strains.

Insectisidal effects of 40 isolates, that were identified at the end of morphological and biochemical tests on Agelastica alni (Coleoptera), Neodiprion sertifer (Hymenoptera) and Hyphantria cunea (Lepidoptera) were detected. These results showed that isolated bacteria were highly effective especially on H. cunea (%50-100). However, same isolates showed 5-55% insecticidal effect on Agelastica alni and 10-80% on Neodiprion sertifer.

Keywords: Bacillus thuringiensis, insecticidal crystal protein, biological control.