M.Sc. Tezi Görüntüleme

Student: Neslihan SARUHAN
Supervisor: Prof. Dr. Asım KADIOĞLU
Department: Biology
Institution: Graduate School of Natural and Applied Sciences
University: Karadeniz Technical University, Turkey
Title of the Thesis: THE INVESTIGATION OF BIOCHEMICAL LEVELS OF POLYAMINES ON LEAF ROLLING DURING DROUGHT STRESS
Level: M.Sc.
Acceptance Date: 4/2/2002
Number of Pages: 55
Registration Number: i1237
Summary:

      The effects of three main polyamines (putrescine, spermidine and spermine) on the degree of leaf rolling, changes in the activity of peroxidase were investigated in Ctenanthe setosa during drought stress. In addition, the effect of stress on the amounts of reducing sugar, the changes in soluble protein, proline amounts in the exogenic polyamine applicated leaves were determined.

      For this purpose, the changes in the degress of rolling leaves were studied morfologically. The amounts of reducing sugar, proline, soluble protein and changes in the activity of peroxidase were spectrophotometrically determined.

      The analysis show that, the exogenic application of polyamines delayed the leaf rolling. It was observed that peroxidase activity in polyamine applicated leaves was decreased according to control ones during drought stress. Also it was determined that putrescine and spermidine were caused an increased in the amount of proline and reducing sugar, in contrast spermine pressed this increase. And it was observed that the level of soluble protein in polyamine applicated plants was increased according to control ones during drought stress.

      Also we observed an increased in the amount of peroxidase, proline, reducing sugar and a decrease in soluble protein in polyamine applicated leaves and control ones during drought stress.

      As a result, reduced leaf rolling by polyamines could be explained with the increase amount of proline, reducing sugar and soluble protein. Also it was seen that peroxidase was not significantly effetual in this study.

      Keywords: Ctenanthe setosa, Polyamine, Peroxidase, Reducing Sugar, Proline, Leaf Rolling, Drought Stress