M.Sc. Tezi Görüntüleme

Student: Ali SEVİM
Supervisor: Yrd. Doç. Dr. Osman Birol ÖZGÜMÜŞ
Department: Biology
Institution: Graduate School of Natural and Applied Sciences
University: Karadeniz Technical University, Turkey
Title of the Thesis: INVESTIGATION OF FECAL COLIFORM POLLUTION OF RIVERS IN TRABZON AND ANTIBIOTIC RESISTANCE PROFILES OF COLIFORM BACTERIA
Level: M.Sc.
Acceptance Date: 4/8/2005
Number of Pages: 84
Registration Number: i1609
Summary:

      In the study, between dates December 2003 to 2004 on the bring of each months, totally 120 water samples were collected from ten rivers. To determine the levels of fecal coliform pollution of the samples, multiple-tube fermentation method was used. The samples producing gas in the lactose media were accepted as positive. IMVIC tests were carried out for the identification of coliform bacteria. To determine antibiotic resistance profiles of isolated bacteria, the disk diffusion method was used. In the study, 12 antibiotic disks belonging 6 different antibiotic classes were used.

      At consequent analysis of water samples collected from rivers in Trabzon, in 119 of collected samples, total coliform levels were determine as 1100 MPN/100 ml and in one sample as 240 MPN / 100 ml. At the result of performed IMVIC tests, total 184 strains belonging to Enterobacteriaceae family were isolated. Species are E. coli, Klebsiella pneumoniae, Klebsiella oxytoca, Citrobacter freundii, Citrobacter koserii, Enterobacter spp. and Proteus vulgaris. All of the isolated bacteria were resistant to at least one antibiotic. The highest resistance with 73% was determined against ampicillin. The other resistance rates are 52% streptomycin, 48% neomycin, 43% gentamicin, 28% tetracycline, 25% kanamycin and trimethoprime, 23% netilmicin, 18% amikacin, 17% nalidixic acid, %13 chloramphenicol and 5% ofloxacin.

Consequently, it was encountered that the high fecal pollution rates was in the streams and, the enterobacteria causing the fecal pollution had high level of antibiotic resistance

      

Keywords: Fecal pollution, Antibiotic resistance, Trabzon, River