M.Sc. Tezi Görüntüleme

      In this study, it was aimed that antibiotic resistance profiles, transferability and TEM-type of β-lactamase genes in ampicillin-resistant (Ampr) Escherichia coli strains by polymerase chain resction (PCR) were investigated in E. coli strains isolated from Rize and its counties.

      In the study, a total of 117 E. coli strains from 51 station and its districts, which were 37 from city network system water and 14 from spring water samples, were isolated and then determined their resistance against 11 different antibiotics. Resistance rates were determined as 48.7% sulbactam-ampicillin, 47%ampicillin, 30.7% ceftazidime, 27.3% cefuroxime, 19.6% trimethoprime-sulfamethoxazole, 17.9% amikacin, 15.3% netilmicin, 13.6% gentamicin, 12.8% tetracycline, 10.2% cefazoline and 2.5% chloramphenicol. As a result of conjugation assays it was observed that ampicillin, tetracycline and trimethoprim-sulfamethoxazole resistances were found to be transferable in rate of % 3.3, % 40 and % 4.3, respectively, and frequency at 4.6x10-10 -10-6 .

      Of 117 E. coli strains, 55 (47%) strains were found to be resistant to ampicillin and, blaTEM genes were determined in seven of them by PCR.

      As a results of the study, there was a high level of antibiotic resistance in the environment and, it was determined that this resistance was to be transferable amongst bacteria, constituting a great threat to public and environmental health.

      Keywords: E. coli, antibiotic resistance, transferable resistance, blaTEM, drinking water.