M.Sc. Tezi Görüntüleme

Student: Nihal KUTLU
Supervisor: Prof. Dr. Asım KADIOĞLU
Department: Biology
Institution: Graduate School of Natural and Applied Sciences
University: Karadeniz Technical University, Turkey
Title of the Thesis: THE INVESTIGATION OF BIOCHEMICAL LEVELS OF EFFECT OF 24-EPIBRASSINOLIDE ON LEAF ROLLING IN Ctenanthe setosa
Level: M.Sc.
Acceptance Date: 2/9/2005
Number of Pages: 46
Registration Number: i1641
Summary:

      In this study effects of brassinosteroid (24-epibarssinolid) hormone, which was sprayed to leaves of Ctenanthe setosa and which was prepared in two different concentrations, on leaf rolling which started after exposure of plants to drought stress, was observed. In addition, reasons of this effect taking place during leaf rolling were investigated in biochemical level for this reason throughout of drought period. Changes in leaf rolling of plant were determined morphologically, however, proline, reducing sugar, total soluble sugar quantities, in the day of the drought had the maximum value, were measured spectrophotometrically. Total nitrogen and total protein content were calculated by the method of Kjeldahl.

As a result of analysis noted that exogenous brassinosteroids delayed the leaf rolling in important level. On the other hand, the concentrations of 0,01 and 0,05 ppm retarded the leaf rolling in same rate was determined.

      Biochemical test results showed that 0,01 ppm EBL increased the prolin content, while 0,05 ppm did not effect in comparison with control (drought). However, it was observed that brassinolides caused an increase in quantities of reducing sugar, total soluble sugar, total nitrogen and total protein in comparison with control ones. It was recorded that 0,05 ppm EBL increased reducing sugar content more than that of 0,01 ppm EBL. In spite of that it was found that difference between degrees increasing in content of soluble sugar, total nitrogen and total protein which were resulted from 0,01 and 0,05 ppm EBL was not statistically significant.

In the light of these results the decrease in the leaf rolling which resulted from brassinosteroids can be explained by taking into account the increases which were observed in the content of proline, reducing sugar, total soluble sugar, total nitrogen and total protein.

      Keywords: Ctenanthe setosa, Drougt Stress, Leaf Rolling, Brassinosteroid, Proline, Reducing Sugar, Total Soluble Sugar, Total Nitrogen, Total Protein