M.Sc. Tezi Görüntüleme

Student:	Ali Adem BAHAR
Supervisor:	Prof. Dr. Zihni DEMİRBAĞ
Department:	Biology
Institution:	Graduate School of Natural and Applied Sciences
University:	Karadeniz Technical University, Turkey

Title of the Thesis:	Investigation of Bacterial Flora and Microbial Control Agents of Oberea linearis l. (Coleoptera: Cerambycidae)
Level:	M.Sc.
Acceptance Date:	25/1/2006
Number of Pages:	107
Registration Number:	i1657

Summary:

Oberea linearis (Coleoptera: Cerambycidae) is very serious pest of hazelnut in Turkey. Up to now, there is no effective biological control against this pest around the world.

The aim of this study is to determine microbial flora of the Oberea linearis and find out an opportunistic pathogen to use are possible biological control agent against this pest. For this purpose, Oberea linearis larvae were collected from Trabzon and Giresun hazelnut fields. The microbial flora of this pest and the microbial potential of bacterial isolates were determined.

As a result, 13 different bacteria were isolated from Oberea linearis larvae. Nine of these bacteria determined and characterized at species level and four of them characterized at genus level. In this study, we determined morphological, physiological and biochemical properties, metabolic enzyme profiles by API20E and Phoenix 1000A panel test systems. 16S rRNA gene sequence analysis was also performed to determine isolates at molecular level. The isolates were identified as Acinetobacter calcoaceticus, Enterobacter aerogenes, Pseudomonas sp., Flavobacterium sp., Microbacterium sp., Enterobacter agglomerans, Xanthomonas sp., Pseudomonas sp., Pseudomonas putida, Stenotrophomonas sp., Enterobacter cancerogenus., Stenotrophomonas maltophilia, Serretia marcescens.

65 % insectisidal activity was determined with Serretia marcescens against Oberea linearis.

Keywords: Oberea linearis, Bacterial flora, Biological control, Molecular bacterial identification, Insect

pathology