M.Sc. Tezi Görüntüleme

Student:	İnci CEVHER
Supervisor:	Prof.Asist. Remziye NALÇACIOĞLU
Department:	Biyoloji
Institution:	Graduate School of Natural and Applied Sciences
University:	Karadeniz Technical University Turkey

Title of the Thesis:	Detectıon Of Amv133 Orf Of Amsacta Mooreı Entomopoxvırus By Homolog Recombınatıon And Consructıon Of Recombınant Vırus
Level:	M.Sc.
Acceptance Date:	6/10/2011
Number of Pages:	53
Registration Number:	i2409

Summary:

Amsacta moorei Entomopoxvirus (AmEV) is an insect virus that belongs to Poxviridae family and Entomopoxviridae subfamily. After analysis of complete genomic sequence of AmEV, ORF AMV133 was suggested to be a putative triacylglyceride lipase gene. It has a high level pathogenic effect on insect pests. Furthermore, AmEV can be used as gene expression and gene therapy vectors, and has great importance as model virus for studying the mechanism of human viruses.

Transfer vector and transitory expression vector was constructed to delete AMV133 gene. Assays were made as two different group. First group is that solely transfer vector was used both transfection and plak assays. Second one is that transfer vector and also transitory expression vector were used in that assays.

AMV133 gene was deleted from AmEV genom by homolog recombination. Consructed recombinant virus was named as AmEV-Lip/gfp. At works which were made, it has been observationed that in second group transfection solution has more gfp. At the same time, second one has more recombinant virus in placque assays. However, constructing recombinant viruses were not increased, and this is also show that lipase is an essential gene for AmEV.

Key Words: AmEV-Lip/gfp, Amsacta moorei Entomopoxvirus (AmEV), lipase, insect virus,

homolog recombination.