M.Sc. Tezi Görüntüleme

Student:	Mustafa CÜCE
Supervisor:	Prof. Dr. Atalay SÖKMEN
Department:	Biyoloji
Institution:	Graduate School of Natural and Applied Sciences
University:	Karadeniz Technical University Turkey

Title of the Thesis:	MICROPROPAGATION OFVACCINIUM ARCTOSTAPHYLOS L. (ERICACEAE) VIA SHOOT-BUD CULTURES
Level:	M.Sc.
Acceptance Date:	1/6/2012
Number of Pages:	63
Registration Number:	i2468

Summary:

This thesis was designed to find out the most ideal growth medium for a rapid and efficient micropropagation of Vaccinium arctostaphylos L. via shoot-bud cultures. Preliminary studies showed that April and May were the best periods for the explant collection. Accordingly, cuttings were taken from naturally growing populations of this plant and lateral shoot-buds of which were used as explant sources. In order to determine the best basal medium for multiple shoot formation, Anderson s Rhododendron, McCown s WoodyPlantMedium (WPM) andMurashige&Skoog s,each supplemented with 1.0/0.1 mg/L zeatin/IBA were tested as basal media, respectively. As a result, WPM was observed to be the most effective basal medium for shoot multiplication.

Subsequent shoot multiplication and development studies were therefore carried out with WPM supplemented with an auxin, indole-3-butyric acid (IBA (0.1 mg/L) and two different cytokinins, namely zeatin and thidiazuron (TDZ) with various doses (0.5, 1.0 and 2.0 mg/L, respectively). After six weeks photoperiod (16/8, dark/light) application, zeatin/IBA combinations were found to be the most suitable for the shoot multiplication and growth. The highest of shoot length with a 106.5 percentage was obtained from the medium supplemented with 1,0/0,1 mg/L Zeatin/IBA whilst the highest increase in the leaf number (141.89%) as well as multiple shoot proliferation (10.55 fold) were obtained from those supplemented with 2,0/0,1 mg/L Zeatin/IBA.

McCown s(WPM)was also employed as rooting medium supplemented with different doses of IBA (0.5 to 8.0 mg/L) togethet with illumination (16/8 photoperiod) or dark regimes. At this stage, the medium supplemented with 0.5 mg/L IBA was proven to be the best rooting growth regulator with 100% rooting success in the presence of 16/8 photoperiod applications.

Key Words: Caucasian Whortleberry, Indole-3-butyric acid, Lateral Shoot-bud, Micropropagation, Thidiazuron,Vaccinium arctostaphylos,Zeatin