| Summary: Chilo Iridescent Virus (CIV) belongs to Iridoviridae family and has large host spectrum because it infects insects belong to Lepidoptera, Diptera, Coleoptera, Hymenoptera and Orthoptera. After the entry of CIV virions to host cells via endocystosis, viral genome is directed to nucleus. CIV of 337L ORF (412 amino asids) encodes glycoprotein beta hormones signal and 295L ORF (1343 amino asids) encodes bipartitate nuclear localization signals. Study of homologous of 337L protein belong to ascoviruses showed that the encoded protein facilitate attachment of virus and beginning of viral infection. Another study on homolog of 295L protein in Frog Virus 3 (FV3) showed that expressed protein plays a role for delivering of viral genome to nucleus. Interaction of these proteins is important in frame of making the viral infection quick and efficient. In this study, 337L and 295L were expressed in Sf9 cells using Bac-to-Bac system containing HA-taq and His-taq respectively. Western Blot analysis showed that 337L and 295L encoded proteins about 46 kDa and 95kDa, respectively. Protein-protein interaction between 337L and 295L proteins were demonstrated with pull down assay by using HA-antibody. The band that has a 46 kDa size was shown. As a result, 337L protein also purified with binding to purified 295L and thus the interaction between 337L and 295L gene products was demonstrated. |
