M.Sc. Tezi Görüntüleme

Student:	Merve TUNÇEL
Supervisor:	Prof. Dr. Sabriye ÇANAKÇI
Department:	Biyoloji
Institution:	Graduate School of Natural and Applied Sciences
University:	Karadeniz Technical University Turkey

Title of the Thesis:	Construction and Characterization of A New Chımeric Xylanase Active At High Temperature and Ph
Level:	M.Sc.
Acceptance Date:	25/9/2014
Number of Pages:	91
Registration Number:	i2837

Summary:

Chimeric xylanase genes were constructed from B. halodurans C-125 and Geobacillus sp. TF16 genes with a method based on DNA shuffling. These chimeric genes were named as GeoInH2CTer, GeoInH2CTer2, GeoIntraH2Int3 and GeoIntraH2Int4. Chimeric genes were cloned into pET28a(+) expression vector. GeoIntraH2Int3 and GeoIntraH2Int4 were succesfully expressed in E. coli BL21 (DE3), but there was no expression for GeoInH2CTer and GeoInH2CTer2. Optimum temperature of GeoIntraH2Int3 and GeoIntraH2Int4 were 60 °C, 50-60 °C and optimum pH of them were 8,0 and 8,0-10,0, respectively.

Key words: Bacillus halodurans C-125, Geobacillus sp. TF16, DNA shuffling, Chimeric gene