Student:	GÖNÜL ALGI
Supervisor:	PROF DR MUSTAFA YAMAN
Department:	Biyoloji
Institution:	Graduate School of Natural and Applied Sciences
University:	Karadeniz Technical University Turkey

Title of the Thesis:	IZOLATION, CHARACTERIZATION OF A MICROSPORIDIUM (Protista) FROM POPLAR LEAF BEETLE AND DİFFERENT POPULATIONS DISTRIBUTION
Level:	M.Sc.
Acceptance Date:	15/6/2015
Number of Pages:	45
Registration Number:	i2937

Summary:

A microsporidium species detected from Poplar leaf beetle (Crepidodera aurata

(Marsham); Coleoptera: Chrysomelidae) which cause important damage in poplar trees in

our country and worldwide was characterized in this study. The adults were studied in

different 10 locality between the years 2013 – 2014. Fresh preparations were dissected and

examined under the light microscope with magnifications 40x-1000x. Isolat 1 dimensions

were measured as 3.66-5.66 x 1.35-2.22 m and isolat 2 2.44-3.55 x 1.25-1.55 m in fresh

preparations dissected. Infection was observed in many tissues and organs such as glands,

malpighian tubules, intestine of host and the life cycle of the microsporidian.

Ultrastructural features of the pathogen were determined with electron microscopy.

According to ultrastructural studies on pathogen spore; this pathogen is monocharyotic and

it has 8 polar filament, 120-125 nm spore wall thickness and 80-95 nm polar filament

dimension. The infection was detected in 7 out of 10 different localities which sampling

found that the rate of infection was found to be 25 percent in some populations. İt is given

for the first time for the literature of the world that a characterization and distribution of

microsporidia pathogen which causes diseases in natural populations of C. aurata in this

thesis.

Key Words: Crepidodera aurata (Marsham), biological control, microsporidium,

characterization, Turkey