M.Sc. Tezi Görüntüleme

Student:	Orcan DEMİRCAN
Supervisor:	Prof. Dr. Sabriye ÇANAKÇI
Department:	Biyoloji
Institution:	Graduate School of Natural and Applied Sciences
University:	Karadeniz Technical University Turkey

Title of the Thesis:	STUDIES ABOUT USING OF SOME BACILLUS STRAINS IN THE BIOREMEDIATION OF PULP BLACK LIQUOR
Level:	M.Sc.
Acceptance Date:	28/6/2019
Number of Pages:	60
Registration Number:	i3648

Summary:

The paper and pulp industry is recognized as one of the largest and also the most polluting industries in the world. Black liquor which is a wastewater with high pH, COD and BOD and produced by paper and pulp industries, is a pollutant which is very dangerous for the environment and causes genotoxic, carcinogenic and clastogenic effects on organisms. In this respect, Bacillus megaterium and Bacillus licheniformis bacteria used in this study are thought to be potential organisms in bioremediation of this wastewater.

It was determined that B. megaterium and B. licheniformis bacteria were removed color by ~11% and ~16% and degraded lignin by ~13% and ~14%, respectively, under optimum conditions and compared to the negative control. Maximal decolorization and degradation was observed after 72 cultivation hours mostly by the six-day optimization studies with red pine pulp black liquor. In addition, the presence of lignolytic potential in these bacteria was investigated with zymography and molecular studies. DyP-type peroxidase enzymes in these bacteria were cloned.

Keywords: Bioremediation, Bacillus licheniformis, Bacillus megaterium, Black liquor,

DyP-type peroxidases